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Ten suggestions to apply the Best Micro-Pipette Practice in microbiology

Equipment Care – Service every 8-12 months (Calibration, greasing parts, seals replacement, cleaning, disinfection, etc.). Tips – Replace tips every transfer (reused tips will carry extra organisms over the next sample). Aspiration Depth – Place tip just below the surface of the liquid (Too deep results in extra organism carry over on the outside of the tip). Slow, fluid motions – Do not allow pipette to “snap” up liquid into the tip (This creates air bubbles in the tip, and risk liquid entering the pipette shaft).

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