N.82 – Sampling viable organisms by microbial active air samplers

Importance of the impaction on agar surface. Effect of jet velocity, nozzle size, nozzle distance from the agar surface, quality of medium, volume of medium inside the culture, expiration date of the plate, moisture content and sterility of the medium, temperature should be considered to obtain the optimization of these variables. These facts are determinative […]

N.81 – The Bluetooth applications to transfer data from the TRIO.BAS microbiological air sampler

APPLICATION NOTE by AROUND LAB NEWS The Bluetooth applications to transfer data from the TRIO.BAS microbiological air sampler.  The Bluetooth applications is an ideal electronic device to transfer the environmental data collected by the TRIO.BAS air samplers. The Barcode collects data from culture plate and location (A1), operator (A2) At the end of sampling, the […]

N.79 – Culture medium and diluent USP 38

Culture media and diluents – Selection of Growth Media in Cleanrooms, RABS, Isolator Microbiology according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments -Page 1201, 1196. – Culture media and diluents The type of medium, liquid or solid, used for sampling or plating microorganisms depends on the procedures and equipment used. […]

N.78 – Microbial isolates identification according USP 38

Identification of microbial isolates Identification according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments -Page 1201 A successful environmental control includes an appropriate level of identification of the flora obtained in sampling. A knowledge of the flora in controlled environments aids in determining the usual microflora anticipated  for the facility […]

N.76 – SOP – Standard Operating Procedure for use, maintenance, calibration of “TRIO.BAS” microbiological air samplers

OBJECT The purpose of this document is to describe the procedure for sampling, and reporting of the viable airborne particulates as part of environmental program, using TRIO.BAS Microbiological Air Sampler. OBJECTIVE This procedure applies to particulate sampling and evaluation of air quality for viable particulates using a Microbiological Air Sampler GLOSSARY Agar, Autoclave, Bacteria, Bioburden, […]

N.75 – Sequential sampling

Sequential sampling with TRIO.BAS DUO and TRIO in Clean Room, Isolators, RABS With TRIO.BAS DUO microbial air sampler (with 2 separated aspirating chambers) and TRIO.BAS TRIO microbial air sampler (with 3 separated aspirating chambers) it is possible to monitor two or three different environments or spots of the same environment, simultaneously. Sequential sampling in Clean […]

N.73 – Air sampler disinfection

Cleaning and disinfection of microbial active air samplers in Clean Room INTRODUCTION All the materials that are used with a microbial air sampler (protective aspirating head, aspirating head, body of the air sampler, bags, etc) should be cleaned and disinfected before the sampling cycles. CLEANING The external part of the air sampler should be treated […]

N.72 –  THE CHOISE AND USE OF AN ACTIVE MICROBIAL AIR SAMPLER

THE CHOISE AND USE OF AN ACTIVE MICROBIAL AIR SAMPLER The microbial air sampler must be carefully chosen because the characteristics of the instrument may impact heavily on usability, on implementing deadlines and consequently the final cost of such activities in terms of buying and managing the facility, man hours and efficiency of operations  The […]