N.6 – Sampling Plan

TRIO

Introduction 

The air sampling plan in check-list format aids both the operator’s supervisor and the operator in remembering all the points required for correct microbiological air sampling. 

 

 1. STAGE OF SAMPLING PLAN  ACTIONS AND COMMENTS 
Date of sampling  
Reason for sampling  
Air Sampler Type  
Accessories  
table tripod  
floor tripod  
remote switch  
additional sterilized heads  
aerosol disinfectant  
Sampling form  
Sampling area  
Sampling location  
Operator’s name  
Sample file  
Time of sampling (0-24 hours)  
Sampling interval  
Volume of air  
Replicate sampling  
Sample identification number  
Type of plate  
Number of people during sampling  
Type of media  
Micro-organisms to be counted  
Micro-organisms to be identified  
HVAC switch on or off  
Temperature of environment  
indoor  
outdoor  
Atmospheric humidity  
In situ head sterilization  

 

 2. CONTACT PLATES OPANING/RE PACKING  ACTIONS AND COMMENTS 
Container used  
Container code  
Disinfectant  
Sterile gloves (clean room)  

 

 3. TRANSPORT TO THE LABORATORY  ACTIONS AND COMMENTS 
Address of laboratory  
Working hours of the laboratory  
Dead-line for sample transport  
Transport case  
Refrigerated case  
Method of transport  
Person responsible  
Means of transport  

 

4.SAMPLE PREPARATION IN LABORATORY  ACTIONS AND COMMENTS 
Is immediate analysis needed?  
Which data are recorded and how?  
Incubation temperature  
Incubation time  
Sample report form details  

Reference

IES-RP-CC023.1-Microorganisms in clean rooms- Institute of Environmental Sciences