N.75 – Sequential sampling

TRIO

Sequential sampling with TRIO.BAS DUO and TRIO in Clean Room, Isolators, RABS

With TRIO.BAS DUO microbial air sampler (with 2 separated aspirating chambers) and TRIO.BAS TRIO microbial air sampler (with 3 separated aspirating chambers) it is possible to monitor two or three different environments or spots of the same environment, simultaneously.

TRIO-BAS-COMPLETO-FRONT-TRIO TRIO-BAS-COMPLETO-FRONT-DUO

Sequential sampling in Clean Room

The sequential program gives the possibility to perform sequential sampling cycles spaced out by several stand-by times, obtaining a more representative microbial air monitoring.

Sequential sampling in isolators

The 3 separated and spaced chambers of the TRIO.BAS model Isolator give the chance to monitor 3 different areas of isolator or environments even if far from each other.

TRIO.BAS-ISOLATOR

Example Protocol of sequential sampling

DUO.BAS DUO microbial air sampler – 100 lts/minute flow rate – Aspiration chambers A end B – Interval time 5 minutes – Total sub-samples 20 – Total time 50 minutes – Total air volume 2000 (1000+1000) litres.

To collect 1000 litres (500 litres per plate) of air, only five aspirating cycles are necessary.

To collect 1000 litres (1000 litres per plate), of air, ten aspirating cycles are necessary.

TRIO.BAS TRIO microbial air sampler – 100 lts/minute flow rate – Aspiration chambers A, B, C – Interval time 5 minutes – Total sub-samples 21 – Total time 35 minutes – Total air volume 2100 (700+700+700) litres

ASPIRATION CHAMBER ASPIRATING CYCLE SUB-SAMPLE INTERVAL TIME (MINUTES) SAMPLING TIME (SECONDS) ASPIRATED VOLUME OF AIR PROGRESSIVE VOLUME OF AIR
A

B

I 1

2

0’ 00” 60 100

100

200
A

B

II 3

4

5’ 00” 60 100

100

200+200=400
A

B

III 5

6

10’ 00” 60 100

100

400+200=600
A

B

IV 7

8

15’ 00” 60 100

100

600+200=800
A

B

V 9

10

20’ 00” 60 100

100

800+200=1000
A

B

VI 11

12

25’ 00” 60 100

100

1000+200=1200
A

B

VII 13

14

30’ 00” 60 100

100

1200+200=1400
A

B

VIII 15

16

35’ 00” 60 100

100

1400+200=1600
A

B

IX 17

18

40’ 00” 60 100

100

1600+200=1800
A

B

X 19

20

45’ 00” 60 100

100

1800+200=2000
ASPIRATION CHAMBER ASPIRATING CYCLE SUB-SAMPLE INTERVAL TIME (MINUTES) SAMPLING TIME (SECONDS) ASPIRATED VOLUME OF AIR PROGRESSIVE VOLUME OF AIR
A

B

C

I 1

2

3

0’ 00” 60 100

100

100

300
A

B

C

II 4

5

6

5’ 00” 60 100

100

100

300+300=600
A

B

C

III 7

8

9

10’ 00” 60 100

100

100

600+300=900
A

B

C

IV 10

11

12

15’ 00” 60 100

100

100

900+300=1200
A

B

C

V 13

14

15

20’ 00” 60 100

100

100

1200+300=1500
A

B

C

VI 16

17

18

25’ 00” 60 100

100

100

1500+300=1800
A

B

C

VII 19

20

21

30’ 00” 60 100

100

100

1800+300=2100