125 - TRIO.BAS Air Sampler - Care, Maintenance, Battery Charging

The TRIO.BAS microbial air samplers should be cleaned at regular intervals. The body of the unit should be treated with mild detergent or ethanol. The stainless steel aspirating head should be cleaned with different type of detergents (exclude chlorine detergent).

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124 - Cleanroom Microbial Air Monitoring with Operative Efficiency by TRIO.BAS Air Sampler

The Cleanroom monitoring is a continuous program to evidentiate control and detect possible excursions in the environment of aseptic manufacturing area. The choice of location of the air samplers and frequency is based on risk evaluation driven by the nature of cleanroom activity, equipments and process workflow.

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123 - Indoor Air Bioaerosol Sampling from Building according NIOSH

This Application Note reports the original method from the technical paper 0800 published on 1998 by NIOSH USA (NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH). After 20 years the method is still a valid and well done document. The new generation of air samplers simpli es the reported sampling process.

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122 - Microbial Air Monitoring suggestions for reference graphic preparation

A speci c graphic to be implemented should be the basis for the interpretation of the microbiological results in non “standardied closed environment”. The graphic is then used as a guide for the environment to be monitored at regular programmed intervals. It is important to consider in a closed space the occupant density, the building characteristics, the humidity for a correct evaluation of the results. The graphic is the most effective method to involve the personell in the Hygiene care.

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121 - Evaluation of Microbial Contamination in Hospital, Clinic, Health Care Premises, etc

The Guide lines for the evaluation of the microbial environmental contamination in the operating rooms of hospital, clinic and health care premises are repported in the document of World Health Organisation (WHO), National Health Service (NHS).

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120 - Correrct use of reference graphic in trends for Microbiological Environmental Data (MED)

The microbiological environmental monitoring data is part of a continuum. Periodic analyses of environmental data should be formally prepared, reviewed by microbiological QA and presented for informational purposers by the production and general personnel responsible for speci c areas, operations and products.

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119 - Expulsion of Droplets during a Sneeze

During a sneeze, millions of tiny droplets of water and mucus are expelled at about 200 miles per hour (100 metres per second). The droplets initially are about 10-100 micrometers in diameter, but they dry rapidly to droplet nuclei of 1-4 micrometres, containing virus particles or bacteria. This is a major means of transmission of several diseases of humans, shown in the table.

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118 - TRIO.BAS Aspiration Cycle “Multimode”

The microbial air sampling can be programmed by sub-samples to obtain results during longer time. All TRIO.BAS air samplers can be programmed for “Delay time”, “Interval time”, “Sampling time”- It is therefore possible to obtain sequential sampling spaced out by several standby times: -Delay time from 1 to 120 minutes. -Sampling intervals up to 100. -Sampling time up to 60 minutes. These performances are very useful for sampling “AT REST” and “IN OPERATION”.

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117 - Airborne Bacteria, Viruses and Spores in poultry premises by High Efficiency Electrostatic Bacterial Air Sampler

It is well known that anytime there are birds infected with pathogenic organisms in a poultry area, these microorganisms are shed in some way and most of them can be recovered from room surfaces or from the air. A high ef ciency, portable electrostatic bacterial air sampler (EBAS) was designed for recovering low levels of bioaerosols in poultry spaces.

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116 - MUSEUM OF MICROBIAL AIR SAMPLERS Villa Cella – Milan

The Museum of Microbial Air Samplers is located in Villa Cella in Milan and presents the instrumentation starting from the prototype of SAS in 1970’ together with the subsequent copies made by competitors up today.

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115 - Protocol of Dressing in Cleanroom

The correct dressing of operators in controlled contamination environment is a key point to guarantee the lowering of the particle and microbial count to requested values. This aim is achieved by adopting a well-de ned procedure. One example is suggested by Philip Austin, resumed in the publication “Modular Cleanroom "Terra Universal Vol. 10. N. 3. It illustrates proper donning step-by-step itinerary.

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114 - The Gamma Irradiation

Gamma rays are a form of electromagnetic radiation-like x-rays, but with higher energy. The primary industrial sources of gamma rays are radionuclide elements such as Cobalt 60, which emit gamma rays during radioactive decay. Gamma rays pass readily through plastics and kill bacteria by breaking the covalent bonds of bacterial DNA. They are measured in units called kiloGrays (kGy). Gamma irradiation provides a number of bene ts in cost and sterility assurance. It can be applied under safe, wellde ned, and controlled operating parameters, and is not a heat- or moisturegenerating process. Consequently, there is no heat stress and condensate drainage or outgassing is not required. Most importantly, there is no residual radioactivity after irradiation.

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