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122 - Microbial Air Monitoring suggestions for reference graphic preparation

A speci c graphic to be implemented should be the basis for the interpretation of the microbiological results in non “standardied closed environment”. The graphic is then used as a guide for the environment to be monitored at regular programmed intervals. It is important to consider in a closed space the occupant density, the building characteristics, the humidity for a correct evaluation of the results. The graphic is the most effective method to involve the personell in the Hygiene care.

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119 - Expulsion of Droplets during a Sneeze

During a sneeze, millions of tiny droplets of water and mucus are expelled at about 200 miles per hour (100 metres per second). The droplets initially are about 10-100 micrometers in diameter, but they dry rapidly to droplet nuclei of 1-4 micrometres, containing virus particles or bacteria. This is a major means of transmission of several diseases of humans, shown in the table.

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118 - TRIO.BAS Aspiration Cycle “Multimode”

The microbial air sampling can be programmed by sub-samples to obtain results during longer time. All TRIO.BAS air samplers can be programmed for “Delay time”, “Interval time”, “Sampling time”- It is therefore possible to obtain sequential sampling spaced out by several standby times: -Delay time from 1 to 120 minutes. -Sampling intervals up to 100. -Sampling time up to 60 minutes. These performances are very useful for sampling “AT REST” and “IN OPERATION”.

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116 - MUSEUM OF MICROBIAL AIR SAMPLERS Villa Cella – Milan

The Museum of Microbial Air Samplers is located in Villa Cella in Milan and presents the instrumentation starting from the prototype of SAS in 1970’ together with the subsequent copies made by competitors up today.

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109 - Agar moisture loss in Petri dish using an impact air sampler system

The optimal growing of colonies on a culture plate is obtained with an agar medium that has the correct volume, the correct level of moisture and is freshly prepared. The agar plates can lose moisture for high temperature during the storage and during the air sampling due to the impact of air on the agar surface. According to the literature the loss should be lower than 10%. It is here reported an example of SOP (Standard Operative procedure) to evaluate the loss of humidity on Petri dish during the air sampling by an active air sampler.

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106 - Suggested procedures for microbial air sampling

In environments with constant characteristics it is easier to provide “Acceptability Levels” to be used for routine work. It is not possible to set the same standards for all applications and the “Acceptability Limits” will vary considerably in different environments.

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105 - Microbial Air sampler positioning

A microbial air sampler should be positioned with different orientations according to the purpose of the test in the considered area (e.g. risk evaluation). Are here reported several applications for the TRIO.BAS microbial air samplers.

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102 - Evaluation of two microbial active air samplers

The aim of this research was to evaluate and compare the results of two microbial air samplers present on the market: the non portable Anderson one stage model and the portable TRIO.BAS MONO system. The air samples were collected in 3 different environments with the two air samplers positioned 1 meter from the other, in absence of personell during the aspiration. The results showed that the variability between the two instruments was not signi cative and was comparable.

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101 - The “physical laws” that regulate the diffusion and deposition of particles in the air

The here repor ted de nitions of few fundamental physical laws can help to understand the mechanism of particle behavior and consider the consequent air sampling difficulties. Brownian motion: As the par ticles migrate through a body of air, random impacts from individual molecules will cause them to veer from course. Drag coefficient: It is the ratio of the force of gravity to the iner tial force on a par ticle in uid. It indicates how a par ticle will resist any force that could cause a change in the par ticle velocity. Smaller par ticles have smaller drag coef cients due to their lesser masses.

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99 - Microbial Air Sampling Collection Efficiency – The number of Stokes

The efficacy of microbial air sampling collection by impact on agar samplers is characterized by a number without dimensions, called the “number of Stokes”. This number, called (d50), indicates the value that refers to the aerodynamic diameter of particulate that are collected by 50%. Hinds (1982) considers the value (d50) as the diameter of particulates that have a bigger diameter and theoretically are all collected.

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98 - The Micro-Organisms in the Air

Of all environmental, air is the simplest one and it occurs in a single phase, gas. Air is not a natural environment for the growth and reproduction of microorganisms. It does not contain necessary amount of moisture and utilizable form of nutrient. In addition to gasses, dust particles and water vapour, air also contains microorganisms. There are vegetative cells and spores of bacteria, fungi and algae, viruses and protozoan cysts.

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97 - News in TRIO.BAS Sampler Software and Hardware

NEW SPECIFICATION (OCT 2016), “Delay” functions is now from 1 minutes to 120 minutes, The range of action of Bluetooth is now about 100 meters

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