25 - European Standard for biological Agents Monitoring in workplace atmosphere
The European Standard EN 14042, April 2003 “Workplace atmospheres – Guide for application and use of procedures for the assessment of exposure to chemical and biological agents” 6.3 “Measurement of airborne micro-organisms and endotoxin”, 6.3.1. “Methods using an impactor as sampler” presents the Table.
Learn more24 - Record of results for microbial air sampling
The dispersion and diffusion of micro-organisms in indoor air is very irregular and influenced by several factors like the presence of air currents, number of people present in the environment, movement of people in the area, electrostatic charges, humidity, temperature, etc. It is clear the correct sampling technique is important. It is imperative to make several tests in the same place, at same time to produce a statistical result that takes an accurate picture of the microbiological conditions of the considered environment. To obtain a statistical result, it is imperative to have a correct record of all data of the sampling cycles.
Learn more23 - Food Glossary according EFSA (European Food Safety Authorities)
An estimate of the amount of a substance in food or drinking water that can be consumed over a lifetime without presenting an appreciable risk to health. It is usually expressed as milligrams of the substance per kilogram of body weight and applies to chemical substances such as food additives, pesticide residues and veterinary drugs.
Learn more22 - Microbial monitoring in closed environment: general suggestions
Viable particles are bacteria, yeast and moulds living microscopic organisms present in the air and on surfaces of the environment. Their testing usually includes the monitoring of – air, – surfaces, – staff. Viable air sampling Gravitational or settling Petri or Contact plates containing sterile growth media are used for sampling.
Learn more21 - Air Sampling Standard According ISO 14698
The FDA Process Analytical Technology (PAT) requires a more efficient and reliable microbiological air monitoring system. The ability to check and to identify possible microbial contamination during the production and process enables companies to take more quick appropriate and immediate measures to avoid economic losses. The new generation of microbiological air samplers and new monitoring procedures improve the efficiency of the measurement of the microbial air quality in clean rooms and controlled areas.
Learn more20 - Legionella aerosol monitoring
Legionnaires’ disease was first recognized as a result of an outbreak of acute pneumonia that occurred at the Convention of the American Legion in Philadelphia USA in 1976. The route of infection has been recognized as the inhalation of small aerosol containing bacteria of the Legionella sp into the lungs of the host animal. The bacterium primarily responsible for this disease is Legionella pneumophila sero-group 1 Pontiac. It can result in typical Legionnaires’ disease an acute pneumonia with low attack rate and relatively high fatality rate or Pontiac fever, a mild non-pneumonia infection with a high attack rate.
Learn more19 - Industrial air handlers microbiological monitoring
The microbiological efficiency of the filters used in buildings is an essential parameter that should be regularly monitored to be sure that the contamination is under control. This Application Note reports a simple method used at 3M Development Laboratory in St. Paul, Minnesota, USA to measure the filter efficiency for fungi of industrial air handlers. The test can help to determine Indoor Air Quality in office buildings.
Learn more18 - Compressed air microbiological monitoring
The present Application Note intends to be a simple Guide Line for the operators involved in the compressed air monitoring applying the ISO 8573-7. The ISO 8573 consists of nine different parts: 1. Contaminants and purity classes. 2. Test methods for aerosol oil content. 3. Test methods for measurement of humidity. 4. Test methods for solid particle count. 5. Test methods for oil vapour and organic solvent content. 6. Test methods for gaseous contaminant content. 7. Test method for viable microbiological contaminant content. 8. Test methods for solid particle content by mass concentration. 9. Test methods for liquid water content.
Learn more17 - Nosocomial Infections: Aspergillosis air sampling
Nosocomial-acquired aspergillosis typically occurs in the setting of treatment for leukaemia or other haematological malignancy. As Aspergillus species are easily found in the environment, it is accepted that aspergillosis disease can be a consequence of exogenous acquisition of the fungus.
Learn more16 - Stainless Steel Aspirating head for microbiological air sampler
The stainless steel sampling heads for Contact Plates (RODAC) or Petri dishes are manufactured of AISI stainless steel and are drilled with holes 1 mm in size. The operators can interchange these heads with no difference in air flow on an air sampler regardless of which head is used, as long the recommended procedures below are followed.
Learn more15 - Trouble shooting in environmental monitoring
The major routes of contamination can be broadly classified as surfaces, water, people and air. Each time that “ALERT LEVEL” and “ACTION LEVEL” are reached, the person responsible for environmental monitoring should know what action must be taken. The reported troubleshooting guide taken from the Document Guide Line No.20 – Effective Microbiological Sampling of Food Processing Environments – by C. & C. gives some useful hints.
Learn more14 - Standard Operative Procedure for Microbial Air Sampling in Clean Room
It is important to organize a correct and clear sampling plan when several clean rooms or controlled contamination areas are to be monitored at the same time for microbial bioburden. To reach this goal it is necessary to have a suitable set of air samplers and a specific SOP that gives to the operators all the information to avoid mistakes and / or misunderstanding. The reported Standard Operating Procedure (SOP) can be used as a guide to be adopted to each individual case.
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