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61 - Choose and comparation of samplers
Active Microbial Air Sampler idea The portable active microbial air sampler collects a pre-determined volume of air that impacts against agar-based growth culture medium contained in a Petri dish or contact plate. The aspiration is produced by a motor-fan. The plate is then incubated at appropriate temperature and the number of colonies (Colony Forming Unit = CFU) per plate are typically reported to 1.000 litres of air.
Learn more60 - Microrganism stress
Effect of Impact stress on microbial recovery on an agar surface of an active air sampler. Microbial stress due to impaction of microorganisms onto an agar collection surface is dif cult to experimentally demonstrate. Recovery and injury due to collection depends not only on the magnitude of the impaction velocity, but also on the degree to which the microorganisms may be embedded in the collection medium.
Learn more59 - Hole impact distribution
Even distribution of air flow on the agar of Petri and Contact dish. The purpose of the test was to verify the regular distribution of the air flow on the surface of agar of a 90 mm Petri dish. The evaluation is performed counting the number of the impact holes produced on the gar surface, after aspiration. AIR SAMPLER TRIO.BAS MINI (100 litres/minute) with s/s aspirating head (Petri dish containing SDA medium)
Learn more58 - Fumigation
The aspiration of bio-aerosol by the TRIO-BAS MINI and TRIO.BAS MONO. The picture shows the aspiration of the aerosol by the smoke test.
Learn more57 - SOP Microbial Compressed Air/Gas Sampling according ISO 8573-7
Object The purpose of this document is to describe the procedure for compressed air / gas sampling, and reporting of the viable airborne particulates as part of environmental program, using TRIO.BAS GAS Microbial Air Sampler. Purpose This procedure applies for sampling and evaluation of compressed air quality for viable particulates using a Microbiological Air Sampler in Clean Room. The purpose is to certify the absence of microorganisms in 1.000 litres of compressed gas/air.
Learn more56 - Microbial Air Sampler with different air flow rate performances comparison
This paper refers to the performances of different microbial active air samplers with different aspiration air flow rate (100, 180 and 200 litres of aspiration air per minute). The purpose is to evaluate the possible different microorganisms collection efficiency.
Learn more55 - TRIO.BAS MINI Microbial Air Sampler Brief Illustrated Instructions
TRIO.BAS MINI Microbial Air Sampler Brief Illustrated Instructions
Learn more54 - Application of MPN in microbial air environmental tests
When the air is aspirated through the aspirating head of the air samplers indicated in the USP 1116 (Slit to agar samplers, Sieve impactors, Centrifugal samplers, Surface Air System) and micro-organisms impact upon the agar surface there is a statistical probability that the micro-organisms may land on top of each other and be visually interpreted as one single colony (CFU).
Learn more53 - The Barcode application in microbial environmental monitoring
The application of bar-codes to the microbiological air monitoring procedures can help save time, to better control the activity of the operator and to achieve a complete traceability of the test.
Learn more52 - Short course of microbiology for environmentalist
Text from “The Society for General Microbiology U.K.”
Learn more51 - Training of personnel in Cleanroom, Isolator, RABS according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments – Page 1195
The personnel performances Good personnel performance plays an essential role in the control of contamination, proper training and supervision are central to contamination control. Aseptic processing is the most critical activity conducted in Microbiological Controlled Environments, and manufacturers must pay close attention to details in all aspects of the endeavor. Rigorous discipline and strict supervision of personnel are essential in order to ensure a level of Environmental quality appropriate for aseptic processing.
Learn more50 - Ten suggestions to apply the Best Micro-Pipette Practice in microbiology
Equipment Care – Service every 8-12 months (Calibration, greasing parts, seals replacement, cleaning, disinfection, etc.). Tips – Replace tips every transfer (reused tips will carry extra organisms over the next sample). Aspiration Depth – Place tip just below the surface of the liquid (Too deep results in extra organism carry over on the outside of the tip). Slow, fluid motions – Do not allow pipette to “snap” up liquid into the tip (This creates air bubbles in the tip, and risk liquid entering the pipette shaft).
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