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49 - Simple tips to facilitate the phenotypic identification of aerobic microorganisms after collection and incubation in Petri dish.
SCOPE OF IDENTIFICATION The purpose of the bacterial air monitoring is not only to count the total number of microorganisms for cubic meter (CFU/cm3), but also to identify the microorganism. STARTING PROTOCOL A. The best phase to pick up a colony from the agar surface for identification is the “LOG” phase (not the “LAG” and “Stationary”), because they are behavior properly being at the top of the health.
Learn more48 - The Bluetooth applications to transfer data from the TRIO.BAS microbiological air sampler.
The Bluetooth applications is an ideal electronic device to transfer the environmental data collected by the TRIO.BAS air samplers.
Learn more47 - Glossary for Microbiological Air Environmental Monitoring
Microbiology according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments – Page 1201, 1202. -Airborne Particulate Count – The total number of particles of a given size per unit volume of air. -Airborne Viable Particulate Count – The recovered number of CFU per unit volume of air. -Air Changes – The frequency per unit of time that the air within a controlled environment is replaced. The air can be recirculated or totally replaced.
Learn more46 - Culture media and diluents – Selection of Growth Media in Cleanrooms, RABS, Isolator
Microbiology according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments -Page 1201, 1196. - Culture media and diluents The type of medium, liquid or solid, used for sampling or plating microorganisms depends on the procedures and equipment used. Any medium used should be evaluated for suitability for the intended purpose. The most commonly used all purpose solid microbiological growth medium is soybean casein digest agar. As previously noted, the medium can be supplemented with chemicals that counteract the effect of various antimicrobials.
Learn more45 - Identification of microbial isolates
Identification according USP 38 – The United States Pharmacopeial – Document (1116) Aseptic Processing Environments – Page 1201 A successful environmental control includes an appropriate level of identification of the flora obtained in sampling. A knowledge of the flora in controlled environments aids in determining the usual microflora anticipated for the facility and in evaluating the effectiveness of the cleaning and sanitation procedures, methods, agents and recovery methods. The information gathered by and identification program can be useful in the investigation of the source of contamination, especially when recommended detection frequency are exceeded.
Learn more44 - Environmental Microbial Data interpretation in aseptic processing.
We report what USP 38 – USP Pharmacopeial Conventions says at page1199 in the Chapter “Further considerations about data interpretation” of the document “Overall Management of a microbiological control program”. ZERO CONTAMINATION “In the high quality environments required for aseptic processing, detection frequency typically is low. As can be seen from the rates recommended in the enclosed table.
Learn more43 - Available microbial air samplers for airborne microorganisms.
The USP 38 – The United States Pharmacopeial Document – Official from May 1, 2015 – presents on page 1200 the list of the most commonly used tools for monitoring aseptic environments. SLIT-TO-AGAR AIR (STA) The unit is powered by an attached source of controllable vacuum. The air intake is obtained through a standardized slit below which is placed a slowly revolving Petri dish that contains a nutrient agar. Airborne particles that have sufficient mass impact the agar surface, and viable microorganisms are allowed to grow. A remote air intake is often used to minimize disturbance of unidirectional airflow.
Learn more42 - Suggested Frequency and Microbial Recovery rate of Sampling for Aseptic Processing Areas
The USP 38 – The United States Pharmacopeial Document – Official from May 1, 2015 – presents on page 1197, Table 2 the “Suggested Frequency of Sampling for Aseptic Processing Areas”.
Learn more41 - Evaluation of different microbial air samplers
To evaluate different microbial air samplers it is useful to read what is written in the USP 38 of the US Pharmacopeial – official from May 1, 2015: “There are no standard methods for air sampling, and available literature indicates that air sampling methods are highly variable. It should not be assumed that similar sample volumes taken by different methods will produce similar rates of recovery.
Learn more40 - Installation and use of TRIO.BAS DUO and TRIO microbial air samplers
The suggested steps to follow for a correct installation and use of the TRIO.BAS DUO and TRIO microbial air samplers.
Learn more39 - Installation and use of TRIO.BAS MONO microbial air sampler
The suggested steps to follow for a correct installation and use of the TRIO.BAS MONO microbial air sampler.
Learn more38 - Installation and use of TRIO.BAS MINI BlueTooth microbial air sampler
The suggested steps to follow for a correct installation and use of the TRIO.BAS MINI BlueTooth microbial air sampler.
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